Evolution of genome organization in Oenococcus oeni revealed by physical and genetic map comparison

Authors

  • Líbia Zé-Zé CEVDI, Instituto Nacional de Saúde Dr. Ricardo Jorge, INSA
  • Ivo M. Chelo Instituto Gulbenkian de Ciência
  • Rogério Tenreiro Universidade de Lisboa, Faculdade de Ciências, Centro de Biodiversidade, Genómica Integrativa e Funcional (BioFIG)

Keywords:

Oenococcus oeni, physical mapping, genomic organization

Abstract

The genomic organization of nine strains of Oenococcus oeni belonging to two previously suggested divergent groups was examined by a top-down approach, including analysis of isolated genes and construction of physical and genetic maps. Genomic sequence data from Oenococcus oeni strain PSU-1 was also examined by a bottom-up approach, using sequence data accessible from Joint Genome Institute, which enabled the confirmation of gene location and assessment of transcription direction. Comparison of genomic maps revealed O. oeni as a homogeneous species and supported the existence of two different genomic groups, although in a too much early phase of divergence to be considered as subspecies. O. oeni genomic organization is characterized by an unusual conserved distribution of the two rrn operons located at least 500 kb apart from the putative chromosome replication origin. Differential degrees of conservation may be observed in O. oeni chromosomes, being the neighbouring region of the replication terminus the most conserved one. Since most of the structural polymorphisms can be correlated to the presence of transposase genes and sites of prophage integration, occurrence of macrodiversity events, as insertions-deletions, duplications or inversions of larger genomic regions seems to be ruled out in O. oeni evolution.

Author Biographies

Líbia Zé-Zé, CEVDI, Instituto Nacional de Saúde Dr. Ricardo Jorge, INSA

CEVDI, Instituto Nacional de Saúde Dr. Ricardo Jorge, INSA

Ivo M. Chelo, Instituto Gulbenkian de Ciência

Instituto Gulbenkian de Ciência

Rogério Tenreiro, Universidade de Lisboa, Faculdade de Ciências, Centro de Biodiversidade, Genómica Integrativa e Funcional (BioFIG)

Universidade de Lisboa, Faculdade de Ciências, Centro de Biodiversidade, Genómica Integrativa e Funcional (BioFIG)

Downloads

Published

2010-09-21

Issue

Section

Research Articles