Cell immobilization of Streptomyces coelicolor : effect on differentiation and actinorhodin production Authors María Teresa López-García Microbiology Section, Department of Functional Biology and IUOPA, School of Medicine, University of Oviedo. Beatriz Rioseras Microbiology Section, Department of Functional Biology and IUOPA, School of Medicine, University of Oviedo. Paula Yagüe Microbiology Section, Department of Functional Biology and IUOPA, School of Medicine, University of Oviedo. José Ramón Álvarez Department of Chemical Engineering and Environmental Technology, University of Oviedo. Ángel Manteca Microbiology Section, Department of Functional Biology and IUOPA, School of Medicine, University of Oviedo Keywords: Streptomyces coelicolor, encapsulation, differentiation, antibiotics, cell death Abstract Streptomycetes are mycelium-forming bacteria that produce two thirds of the clinically relevant secondary metabolites. Despite the fact that secondary metabolite production is activated at specific developmental stages of the Streptomyces spp. life cycle, different streptomycetes show different behaviors, and fermentation conditions need to be optimized for each specific strain and secondary metabolite. Cell-encapsulation constitutes an interesting alternative to classical fermentations, which was demonstrated to be useful in Streptomyces, but development under these conditions remained unexplored. In this work, the influence of cell-encapsulation in hyphae differentiation and actinorhodin production was explored in the model Streptomyces coelicolor strain. Encapsulation led to a delay in growth and to a reduction of mycelium density and cell death. The high proportion of viable hyphae duplicated extracellular actinorhodin production in the encapsulated cultures with respect to the non-encapsulated ones. [Int Microbiol 2014; 17(2):75-80]Keywords: Streptomyces coelicolor · encapsulation · differentiation · antibiotics · cell death Downloads PDF Issue Vol. 17 No. 2 (2014) Section Research Articles License Submission of a manuscript to International Microbiology implies: that the work described has not been published before, including publication in the World Wide Web (except in the form of an Abstract or as part of a published lecture, review, or thesis); that it is not under consideration for publication elsewhere; that all the coauthors have agreed to its publication. The corresponding author signs for and accepts responsability for releasing this material and will act on behalf of any and all coauthors regarding the editorial review and publication process.If an article is accepted for publication in International Microbiology, the authors (or other copyright holder) must transfer to the journal the right–not exclusive–to reproduce and distribute the article including reprints, translations, photographic reproductions, microform, electronic form (offline, online) or any other reproductions of similar nature. Nevertheless, all article in International Microbiology will be available on the Internet to any reader at no cost. The journal allows users to freely download, copy, print, distribute, search, and link to the full text of any article, provided the authorship and source of the published article is cited. The copyright owner's consent does not include copying for new works, or resale. In these cases, the specific written permission of International Microbiology must first be obtained.Authors are requested to create a link to the published article on the journal's website. The link must be accompanied by the following text: "The original publication is available on LINK at <http://www.im.microbios.org>. Please use the appropiate URL for the article in LINK. Articles disseminated via LINK are indexed, abstracted, and referenced by many abstracting and information services, bibliographic networks, subscription agencies, library networks, and consortia.
