The phylogeny of uptake hydrogenases in Frankia Authors Melakeselam Leul Department of Plant Physiology, Umeå Plant Science Centre (UPSC), Umeå University, Umeå Philippe Normand Federal Research Institute (IFR) 41 Bioenvironment and Health, Mixed Unit of Research (UMR) 5557, Department of Microbial Ecology, University of Lyon, Lyon Anita Sellstedt Department of Plant Physiology, Umeå Plant Science Centre (UPSC), Umeå University, Umeå Keywords: Frankia, lateral gene transfer (LGT), hydrogenase synton, Frankia cluster Abstract Uptake hydrogenase is an enzyme that is believed to be beneficial for nitrogen fixation in bacteria. Recent studies have shown that there are two sets of uptake hydrogenase genes, named synton #1 and #2, in Frankia sp. Here, we have made a phylogenetic analysis of the structural subunits of hydrogenase synton #1 and #2, which showed a distinct clustering pattern between Frankia strains isolated from different host plants and non-Frankia organisms. The structural subunits of hydrogenase synton #1 of Frankia sp. CpI1, Frankia alni ACN14a and AvCI1 were grouped together while those of Frankia sp. CcI3, KB5, UGL140104 and UGL011102 formed another group. The structural subunits of hydrogenase synton #2 of F. alni ACN14a, Frankia sp. CcI3 and BCU110501 grouped together, but Frankia sp. KB5, CpI1, F. alni ArI3 and AvCI1 formed another group. The structural subunits of hydrogenase synton #1 and #2 of Frankia sp. EAN1pec were more closely related with those of non-Frankia bacteria Streptomyces avermitilis and Anaeromyxobacter sp., respectively, than they were to other Frankia strains, which suggested the occurrence of lateral gene transfer (LGT) involving these organisms. In addition, the accessory Hyp proteins of hydrogenase synton #1 and hydrogenase synton #2 of F. alni ACN14a and Frankia sp. CcI3 were shown to be phylogenetically more related to each other than they were to Frankia EAN1pec. Author Biographies Melakeselam Leul, Department of Plant Physiology, Umeå Plant Science Centre (UPSC), Umeå University, Umeå Department of Plant Physiology, Umeå Plant Science Centre (UPSC), Umeå University, Umeå Philippe Normand, Federal Research Institute (IFR) 41 Bioenvironment and Health, Mixed Unit of Research (UMR) 5557, Department of Microbial Ecology, University of Lyon, Lyon Federal Research Institute (IFR) 41 Bioenvironment and Health, Mixed Unit of Research (UMR) 5557, Department of Microbial Ecology, University of Lyon, Lyon Anita Sellstedt, Department of Plant Physiology, Umeå Plant Science Centre (UPSC), Umeå University, Umeå Department of Plant Physiology, Umeå Plant Science Centre (UPSC), Umeå University, Umeå Downloads PDF Published 2010-09-20 Issue Vol. 12 No. 1 (2009) Section Research Articles License Submission of a manuscript to International Microbiology implies: that the work described has not been published before, including publication in the World Wide Web (except in the form of an Abstract or as part of a published lecture, review, or thesis); that it is not under consideration for publication elsewhere; that all the coauthors have agreed to its publication. The corresponding author signs for and accepts responsability for releasing this material and will act on behalf of any and all coauthors regarding the editorial review and publication process.If an article is accepted for publication in International Microbiology, the authors (or other copyright holder) must transfer to the journal the right–not exclusive–to reproduce and distribute the article including reprints, translations, photographic reproductions, microform, electronic form (offline, online) or any other reproductions of similar nature. Nevertheless, all article in International Microbiology will be available on the Internet to any reader at no cost. The journal allows users to freely download, copy, print, distribute, search, and link to the full text of any article, provided the authorship and source of the published article is cited. The copyright owner's consent does not include copying for new works, or resale. In these cases, the specific written permission of International Microbiology must first be obtained.Authors are requested to create a link to the published article on the journal's website. The link must be accompanied by the following text: "The original publication is available on LINK at <http://www.im.microbios.org>. Please use the appropiate URL for the article in LINK. Articles disseminated via LINK are indexed, abstracted, and referenced by many abstracting and information services, bibliographic networks, subscription agencies, library networks, and consortia.
