PCR: una nueva herramienta para el estudio de hongos ectomicorrícicos
V. Llorens, María Paz Martín Esteban, Elena Hidalgo Hernando
Resum
PCR: a new tool for the study of ectomycorrhizal fungi. PCR (Polymerase
Chain Reaction) is a simple technique that allows the specific amplification of particular regions
of the genome of living organisms to facilitate their study. In the last few years, this and other
complementary techniques have opened the field of the molecular mycology, whose Applications in detection, identification and c1assification of fungi are increasing every day. In this article, the
first of a series dealing with the Molecular Techniques that can be used in Mycology, we explain
the basic principies of both the PCR and two other complementary techniques commonly used
for the molecular analysis of the amplified regions: RFLPs (Restriction Fragment Length
Polymorphisms) and sequencing (determination ofthe primary structure ofthe nucleic acids).
Chain Reaction) is a simple technique that allows the specific amplification of particular regions
of the genome of living organisms to facilitate their study. In the last few years, this and other
complementary techniques have opened the field of the molecular mycology, whose Applications in detection, identification and c1assification of fungi are increasing every day. In this article, the
first of a series dealing with the Molecular Techniques that can be used in Mycology, we explain
the basic principies of both the PCR and two other complementary techniques commonly used
for the molecular analysis of the amplified regions: RFLPs (Restriction Fragment Length
Polymorphisms) and sequencing (determination ofthe primary structure ofthe nucleic acids).
Text complet: Text complet
Aquesta obra és subjecta —llevat que s'indiqui el contrari en el text, en les fotografies o en altres il·lustracions— a una llicència Reconeixement - No comercial - Sense obres derivades 3.0 Espanya de Creative Commons, el text complet de la qual es pot consultar a http://creativecommons.org/licenses/by-nc-nd/3.0/es/deed.ca. Així, doncs, s'autoritza al públic en general a reproduir, distribuir i comunicar l'obra sempre que se'n reconegui l'autoria i l'entitat que la publica i no se'n faci un ús comercial ni cap obra derivada.
ISSN: 2014-0452 (edició electrònica); 1135-1225 (edició impresa).