HeLa-cell adherence patterns and actin aggregation of enteropathogenic Escherichia coli (EPEC) and Shiga-toxin-producing E. coli (STEC) strains carrying different eae and tir alleles

Authors

  • Azucena Mora Department of Microbiology and Parasitology, Faculty of Veterinary Science, University of Santiago de Compostela, Lugo, Spain
  • Miguel Blanco Department of Microbiology and Parasitology, Faculty of Veterinary Science, University of Santiago de Compostela, Lugo, Spain
  • Denise Yamamoto Department of Microbiology, Immunology and Parasitology, São Paulo School of Medicine, Federal University of São Paulo, Brazil
  • Ghizlane Dahbi Department of Microbiology and Parasitology, Faculty of Veterinary Science, University of Santiago de Compostela, Lugo, Spain
  • Jesús E. Blanco Department of Microbiology and Parasitology, Faculty of Veterinary Science, University of Santiago de Compostela, Lugo, Spain
  • Cecilia López Department of Microbiology and Parasitology, Faculty of Veterinary Science, University of Santiago de Compostela, Lugo, Spain
  • María P. Alonso Unit of Microbiology, Calde Xeral Hospital, Lugo, Spain
  • Mônica A. M. Vieira Department of Microbiology, Immunology and Parasitology, São Paulo School of Medicine, Federal University of São Paulo, Brazil
  • Rodrigo T. Hernandes Department of Microbiology, Immunology and Parasitology, São Paulo School of Medicine, Federal University of São Paulo, Brazil
  • Cecilia M. Abe Laboratory of Bacteriology, Butantan Institute, São Paulo, Brazil
  • Roxane M. F. Piazza Laboratory of Bacteriology, Butantan Institute, São Paulo, Brazil
  • David W. Lacher Division of Molecular Biology, Center for Food Safety and Applied Nutrition, US Food and Drug Administration, Laurel, MD, USA
  • Waldir P. Laboratory of Bacteriology, Butantan Ins Elias
  • Tania A. T. Gomes Department of Microbiology, Immunology and Parasitology, São Paulo School of Medicine, Federal University of São Paulo, Brazil
  • Jorge Blanco Department of Microbiology and Parasitology, Faculty of Veterinary Science, University of Santiago de Compostela, Lugo, Spain

Keywords:

enteropathogenic E. coli, Shiga-toxin-producing E. coli, HeLa-cell adherence, intimin

Abstract

A collection of 69 eae-positive strains expressing 29 different intimin types and eight tir alleles was characterized with respect to their adherence patterns to HeLa cells, ability to promote actin accumulation in vitro, the presence of bfpA alleles in positive strains, and bundle-forming pilus (BFP) expression. All of the nine typical enteropathogenic Escherichia coli (tEPEC) studied harbored the enteropathogenic E. coli adherence factor (EAF) plasmid, as shown by PCR and/or EAF probe results. In addition, they were positive for bfpA, as shown by PCR, and BFP expression, as confirmed by immunofluorescence (IFL) and/or immunoblotting (IBL) assays. Localized adherence (LA) was exclusively displayed by those nine tEPEC, while localized-adherence-like (LAL) was the most frequent pattern among atypical EPEC (aEPEC) and Shiga-toxinproducing E. coli (STEC). All LA and LAL strains were able to cause attaching and effacing (AE) lesions, as established by means of the FAS test. There was a significant association between the presence of tir allele α1 and bfpA-positive strains, and consequently, with the LA pattern. However, intimin type or bfpA was not associated with the adherence pattern displayed in HeLa cells. Among the eight bfpA alleles detected, a new type (β10; accession number FN391178) was identified in a strain of serotype O157:H45, and a truncated variant (β3.2-t; accession number FN 391181) in four strains belonging to different pathotypes. [Int Microbiol 2009; 12(4):243-251]

Author Biographies

Azucena Mora, Department of Microbiology and Parasitology, Faculty of Veterinary Science, University of Santiago de Compostela, Lugo, Spain

Department of Microbiology and Parasitology, Faculty of Veterinary Science, University of Santiago de Compostela, Lugo, Spain

Miguel Blanco, Department of Microbiology and Parasitology, Faculty of Veterinary Science, University of Santiago de Compostela, Lugo, Spain

Department of Microbiology and Parasitology, Faculty of Veterinary Science, University of Santiago de Compostela, Lugo, Spain

Denise Yamamoto, Department of Microbiology, Immunology and Parasitology, São Paulo School of Medicine, Federal University of São Paulo, Brazil

Department of Microbiology, Immunology and Parasitology, São Paulo School of Medicine, Federal University of São Paulo, Brazil

Ghizlane Dahbi, Department of Microbiology and Parasitology, Faculty of Veterinary Science, University of Santiago de Compostela, Lugo, Spain

Department of Microbiology and Parasitology, Faculty of Veterinary Science, University of Santiago de Compostela, Lugo, Spain

Jesús E. Blanco, Department of Microbiology and Parasitology, Faculty of Veterinary Science, University of Santiago de Compostela, Lugo, Spain

Department of Microbiology and Parasitology, Faculty of Veterinary Science, University of Santiago de Compostela, Lugo, Spain

Cecilia López, Department of Microbiology and Parasitology, Faculty of Veterinary Science, University of Santiago de Compostela, Lugo, Spain

Department of Microbiology and Parasitology, Faculty of Veterinary Science, University of Santiago de Compostela, Lugo, Spain

María P. Alonso, Unit of Microbiology, Calde Xeral Hospital, Lugo, Spain

Unit of Microbiology, Calde Xeral Hospital, Lugo, Spain

Mônica A. M. Vieira, Department of Microbiology, Immunology and Parasitology, São Paulo School of Medicine, Federal University of São Paulo, Brazil

Department of Microbiology, Immunology and Parasitology, São Paulo School of Medicine, Federal University of São Paulo, Brazil

Rodrigo T. Hernandes, Department of Microbiology, Immunology and Parasitology, São Paulo School of Medicine, Federal University of São Paulo, Brazil

Department of Microbiology, Immunology and Parasitology, São Paulo School of Medicine, Federal University of São Paulo, Brazil

Cecilia M. Abe, Laboratory of Bacteriology, Butantan Institute, São Paulo, Brazil

Laboratory of Bacteriology, Butantan Institute, São Paulo, Brazil

Roxane M. F. Piazza, Laboratory of Bacteriology, Butantan Institute, São Paulo, Brazil

Laboratory of Bacteriology, Butantan Institute, São Paulo, Brazil

David W. Lacher, Division of Molecular Biology, Center for Food Safety and Applied Nutrition, US Food and Drug Administration, Laurel, MD, USA

Division of Molecular Biology, Center for Food Safety and Applied Nutrition, US Food and Drug Administration, Laurel, MD, USA

Waldir P. Laboratory of Bacteriology, Butantan Ins Elias

Laboratory of Bacteriology, Butantan Institute, São Paulo, Brazil

Tania A. T. Gomes, Department of Microbiology, Immunology and Parasitology, São Paulo School of Medicine, Federal University of São Paulo, Brazil

Department of Microbiology, Immunology and Parasitology, São Paulo School of Medicine, Federal University of São Paulo, Brazil

Jorge Blanco, Department of Microbiology and Parasitology, Faculty of Veterinary Science, University of Santiago de Compostela, Lugo, Spain

Department of Microbiology and Parasitology, Faculty of Veterinary Science, University of Santiago de Compostela, Lugo, Spain

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Published

2010-01-21

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Section

Research Articles