International Microbiology, Vol 11, No 3 (2008)

Production of two novel laccase isoforms by a thermotolerant strain of Pycnoporus sanguineus isolated from an oil-polluted tropical habitat

Edgar Dantán-González, Odón Vite-Vallejo, Claudia Martínez-Anaya, Mónica Méndez-Sánchez, María C. González, Laura A. Palomares, Jorge Folch-Mallol


A thermotolerant and halotolerant strain of Pycnoporus sanguineus was isolated from an oil-polluted site in a tropical area located in Veracruz, Mexico. This strain was able to grow at 47ºC and in culture medium containing 500 mM NaCl. The strain was also tolerant to the presence of 30,000 ppm of crude Maya oil. A 68-kDa protein purified submerged cultures exhibited laccase activity towards 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS), guaiacol, syringaldazine and o-dianisidine, for which it presented the highest affinity (Km = 43 µM). Two-dimensional gel electrophoresis analysis showed that, unusual for laccases, the enzyme has two active isoforms, with isoelectric points of 7.00 and 7.08. The purified enzyme showed high thermostability, retaining 40% of its original activity after 3 h at 60ºC. This property seems to correlate with a long “shelf-life”, given that at 40ºC enzyme activity was only gradually lost over a 5-day period incubation. Both, the fungus and its laccase aer likely to have high potential for biotechnological applications.

Full Text: PDF

Sello de Calidad FECYT



5-year Impact Factor of International Microbiology is 2,10.

The journal is covered in several leading abstracting and indexing databases, including the following ones: AFSA Marine Biotechnology Abstracts; Biological Abstracts; Biotechnology Research Abstracts; BIOSIS Previews; CAB Abstracts; Chemical Abstracts; Current Contents–Agriculture, Biology & Environmental Sciences; EBSCO; Embase; Food Science and Technology Abstracts; Google Scholar; IEDCYT; IBECS; Latíndex; MedBioWorld; PubMed; SciELO-Spain; Science Citation Index Expanded; Scopus


Creative Commons License

This work, including photographs and other illustrations, unless the contrary is indicated, is subject to an Attributions–Non-Commercial–ShareAlike 3.0 Creative Commons License, the full text of which can be consulted at You are free to share, copy, distribute and transmit the work provided that the author is credited and reuse of the material is restricted to non-commercial purposes only and that if you alter, transform, or build upon this work, you may distribute the resulting work only under the same or similar license to this one. 


Copyright ® 2013 by Spanish Society for Microbiology (SEM)