Saccharomyces cerevisiae Rds2 transcription factor involvement in cell wall composition and architecture

Authors

  • Inmaculada Moreno GMCA Research Group, Department of Microbiology and Ecology, Faculty of Pharmacy, University of Valencia, Spain
  • Nunzia Tutrone GMCA Research Group, Department of Microbiology and Ecology, Faculty of Pharmacy, University of Valencia, Spain
  • Rafael Sentandreu GMCA Research Group, Department of Microbiology and Ecology, Faculty of Pharmacy, University of Valencia, Spain
  • Eulogio Valentín GMCA Research Group, Department of Microbiology and Ecology, Faculty of Pharmacy, University of Valencia, Spain

Abstract

Although the cell wall is very important in yeasts, relatively little is known about the relationship between its structure and function. In Saccharomyces cerevisiae, a family of 55 transcription factor proteins unique to fungi, so-called zinc cluster proteins, has been described. Of these, Rds2 has been identified as an activator/inhibitor of gluconeogenesis. However, previous studies have pointed out additional roles for this protein, specifically, in the modulation of cell-wall architecture and drug sensitivity. In this work, evidence regarding the role of Rds2 as a regulator of cell-wall architecture and composition is presented based on phenotypical analysis of the cell walls prepared from a S. cerevisiae Rds2 mutant strain. Analyses of the sensitivity of this rds2Δ mutant to different drugs and to osmotic stress showed that Rds2 is indeed involved in the drug-sensitivity response and plays a role in determining osmotic sensitivity. [Int Microbiol 2008; 11(1): 57-63]

Author Biographies

Inmaculada Moreno, GMCA Research Group, Department of Microbiology and Ecology, Faculty of Pharmacy, University of Valencia, Spain

GMCA Research Group, Department of Microbiology and Ecology, Faculty of Pharmacy, University of Valencia, Spain

Nunzia Tutrone, GMCA Research Group, Department of Microbiology and Ecology, Faculty of Pharmacy, University of Valencia, Spain

GMCA Research Group, Department of Microbiology and Ecology, Faculty of Pharmacy, University of Valencia, Spain

Rafael Sentandreu, GMCA Research Group, Department of Microbiology and Ecology, Faculty of Pharmacy, University of Valencia, Spain

GMCA Research Group, Department of Microbiology and Ecology, Faculty of Pharmacy, University of Valencia, Spain

Eulogio Valentín, GMCA Research Group, Department of Microbiology and Ecology, Faculty of Pharmacy, University of Valencia, Spain

GMCA Research Group, Department of Microbiology and Ecology, Faculty of Pharmacy, University of Valencia, Spain

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Published

2010-01-19

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Research Articles