Heterologous protective immunization elicited in mice by Pasteurella multocida fur ompH Authors M. Elena Garrido Department of Genetics and Microbiology and Animal Health Research Center (CReSA), Autonomous University of Barcelona, Bellaterra, Spain Montserrat Bosch Department of Genetics and Microbiology, Autonomous University of Barcelona, Bellaterra, Spain Animal Conservation Genetics-IRTA, Cabrils, Spain Anna Bigas Department of Genetics and Microbiology, Autonomous University of Barcelona, Bellaterra, Spain Ignacio Badiola Animal Health Research Center (CReSA), Autonomous University of Barcelona, Bellaterra, Spain Jordi Barbé Department of Genetics and Microbiology and Animal Health Research Center (CReSA), Autonomous University of Barcelona, Bellaterra, Spain Montserrat Llagostera Department of Genetics and Microbiology and Animal Health Research Center (CReSA), Autonomous University of Barcelona, Bellaterra, Spain Keywords: Pasteurella multocida, vaccine, gene fur, gene ompH, iron-regulated outer membrane proteins (IROMPs) Abstract Different strategies have been developed to produce vaccines against Pasteurella multocida. The approach described herein involves overexpression on the bacterial cell surface of Fur-regulated IROMPs (iron-regulated outer-membrane proteins). Accordingly, the ability of fur mutants to promote heterologous protection was examined in a Swiss mouse animal model. Two fur mutants derived from P. multocida were isolated, one of which was also defective in the OmpH protein. In mice challenged with virulent P. multocida, outer-membrane protein (OMP) extracts of fur cells conferred the same protection as obtained with wild-type cells grown in iron-depleted medium. Total protection was achieved with 40 μg of OMP extract from the fur ompH mutant. Mice administered heat-inactivated fur ompH cells were 60% cross-protected. The presence of a galE mutation in these cells did not further increase the protection level. Additionally, cell disruption by sonication provoked a higher level of protection than conferred by heat-treated cells. Taken together, the results showed that P. multocida fur ompH cells offer a simple and suitable approach for cross-protecting animals against infection with P. multocida. [Int Microbiol 2008; 11(1):17-24] Author Biographies M. Elena Garrido, Department of Genetics and Microbiology and Animal Health Research Center (CReSA), Autonomous University of Barcelona, Bellaterra, Spain Department of Genetics and Microbiology and Animal Health Research Center (CReSA), Autonomous University of Barcelona, Bellaterra, Spain Montserrat Bosch, Department of Genetics and Microbiology, Autonomous University of Barcelona, Bellaterra, Spain Animal Conservation Genetics-IRTA, Cabrils, Spain Department of Genetics and Microbiology, Autonomous University of Barcelona, Bellaterra, Spain Animal Conservation Genetics-IRTA, Cabrils, Spain Anna Bigas, Department of Genetics and Microbiology, Autonomous University of Barcelona, Bellaterra, Spain Department of Genetics and Microbiology, Autonomous University of Barcelona, Bellaterra, Spain Ignacio Badiola, Animal Health Research Center (CReSA), Autonomous University of Barcelona, Bellaterra, Spain Animal Health Research Center (CReSA), Autonomous University of Barcelona, Bellaterra, Spain Jordi Barbé, Department of Genetics and Microbiology and Animal Health Research Center (CReSA), Autonomous University of Barcelona, Bellaterra, Spain Department of Genetics and Microbiology and Animal Health Research Center (CReSA), Autonomous University of Barcelona, Bellaterra, Spain Montserrat Llagostera, Department of Genetics and Microbiology and Animal Health Research Center (CReSA), Autonomous University of Barcelona, Bellaterra, Spain Department of Genetics and Microbiology and Animal Health Research Center (CReSA), Autonomous University of Barcelona, Bellaterra, Spain Downloads PDF Published 2010-01-19 Issue Vol. 11 No. 1 (2008) Section Research Articles License Submission of a manuscript to International Microbiology implies: that the work described has not been published before, including publication in the World Wide Web (except in the form of an Abstract or as part of a published lecture, review, or thesis); that it is not under consideration for publication elsewhere; that all the coauthors have agreed to its publication. 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