Characterization of the Yarrowia lipolytica YlSRP72 gene, a component of the yeast signal recognition particle Authors Lorena Ruiz-Pavón Department of Microbiology and Genetics, CIETUS, IMB/CSIC, University of Salamanca, Spain Ángel Domínguez Department of Microbiology and Genetics, CIETUS, IMB/CSIC, University of Salamanca, Spain Keywords: Yarrowia lipolytica, signal recognition particle, protein secretion, gene SRP72 Abstract The Yarrowia lipolytica SRP72 gene product (YlSRP72), a homolog of the 72-kDa subunit of the mammalian SRP, encodes a putative protein of 602 amino acids. Northern blot analysis revealed a unique YlSRP72-specific transcript of 1.8 kb. The deduced amino acid sequence showed higher identities with the Srp72 proteins of euascomycetes than with hemiascomycetes. Chromosomal hybridization experiments showed that the YlSRP72 gene is located in chromosome V of the standard E150 strain of Y. lipolytica. Fluorescent microscopy revealed that the YlSRP72-GFP fusion protein was expressed in the cytoplasm and nucleus. The YlSRP72 gene was interrupted by the pop-out method; however, deletion of the gene proved to be lethal. This is in contrast to the results described for the Saccharomyces cerevisiae SRP72 gene, which is not essential for cell growth, and supports our previous finding with another component of the yeast recognition particle, YlSEC65. The present work suggests that SRP-dependent targeting is the main secretory pathway in Y. lipolytica, as has been described for higher eukaryotes. [Int Microbiol 2007; 10(4):283-289] Author Biographies Lorena Ruiz-Pavón, Department of Microbiology and Genetics, CIETUS, IMB/CSIC, University of Salamanca, Spain Department of Microbiology and Genetics, CIETUS, IMB/CSIC, University of Salamanca, Spain Ángel Domínguez, Department of Microbiology and Genetics, CIETUS, IMB/CSIC, University of Salamanca, Spain Department of Microbiology and Genetics, CIETUS, IMB/CSIC, University of Salamanca, Spain Downloads PDF Published 2010-01-21 Issue Vol. 10 No. 4 (2007) Section Research Articles License Submission of a manuscript to International Microbiology implies: that the work described has not been published before, including publication in the World Wide Web (except in the form of an Abstract or as part of a published lecture, review, or thesis); that it is not under consideration for publication elsewhere; that all the coauthors have agreed to its publication. The corresponding author signs for and accepts responsability for releasing this material and will act on behalf of any and all coauthors regarding the editorial review and publication process.If an article is accepted for publication in International Microbiology, the authors (or other copyright holder) must transfer to the journal the right–not exclusive–to reproduce and distribute the article including reprints, translations, photographic reproductions, microform, electronic form (offline, online) or any other reproductions of similar nature. Nevertheless, all article in International Microbiology will be available on the Internet to any reader at no cost. The journal allows users to freely download, copy, print, distribute, search, and link to the full text of any article, provided the authorship and source of the published article is cited. The copyright owner's consent does not include copying for new works, or resale. In these cases, the specific written permission of International Microbiology must first be obtained.Authors are requested to create a link to the published article on the journal's website. The link must be accompanied by the following text: "The original publication is available on LINK at <http://www.im.microbios.org>. Please use the appropiate URL for the article in LINK. Articles disseminated via LINK are indexed, abstracted, and referenced by many abstracting and information services, bibliographic networks, subscription agencies, library networks, and consortia.
