Co-occurrence of viral and bacterial pathogens in disease outbreaks affecting newly cultured sparid fish

Authors

  • Esther García-Rosado Department of Microbiology, University of Málaga. Málaga, Spain
  • Irene Cano Department of Microbiology, University of Málaga. Málaga, Spain Institute for Marine Sciences, CSIC, Puerto Real, Cádiz, Spain
  • Beatriz Martín-Antonio Department of Microbiology, University of Málaga. Málaga, Spain
  • Alejandro Labella Department of Microbiology, University of Málaga. Málaga, Spain
  • Manuel Manchado IFAPA El Toruño Center, Junta de Andalucía, El Puerto de Santa María, Cádiz, Spain
  • M. Carmen Alonso Department of Microbiology, University of Málaga. Málaga, Spain
  • Dolores Castro Department of Microbiology, University of Málaga. Málaga, Spain
  • Juan J. Borrego Department of Microbiology, University of Málaga. Málaga, Spain

Keywords:

Vibrio spp., Photobacterium damselae subsp. damselae, Virus, VNNV, VHSV, sparid fish, co-occurrence of pathogens

Abstract

Several microbial disease outbreaks in farm stocks of newly cultured sparid fish species, such as common seabream, redbanded seabream, and white seabream, were recorded from 2004 to 2006. This study describes the isolation and characterization of the potential causative agents, either bacteria or viruses, of these outbreaks. The isolated bacterial strains were characterized according to traditional taxonomical analyses and sequencing of a 16S rDNA fragment. Most bacteria were identified as Vibrio spp. and Photobacterium damselae subsp. damselae. The development of cytopathic effects (CPE) on different fish cell lines, the application of specific nested-PCR tests for infectious pancreatic necrosis virus (IPNV), viral nervous necrosis virus (VNNV) and viral hemorrhagic septicemia virus (VHSV), and subsequent sequence analyses were used for virus detection and identification. VNNV, related to the striped jack neural necrosis virus (SJNNV) genotype, and VHSV, related to the genotype Ia, were the only viruses detected. VNNV was isolated from the three fish species under study in five different outbreaks, whereas VHSV was isolated from common seabream and white seabream during two of these outbreaks. IPNV was not detected in any case. [Int Microbiol 2007; 10(3):193-199]

Author Biographies

Esther García-Rosado, Department of Microbiology, University of Málaga. Málaga, Spain

Department of Microbiology, University of Málaga. Málaga, Spain

Irene Cano, Department of Microbiology, University of Málaga. Málaga, Spain Institute for Marine Sciences, CSIC, Puerto Real, Cádiz, Spain

Department of Microbiology, University of Málaga. Málaga, Spain Institute for Marine Sciences, CSIC, Puerto Real, Cádiz, Spain

Beatriz Martín-Antonio, Department of Microbiology, University of Málaga. Málaga, Spain

Department of Microbiology, University of Málaga. Málaga, Spain

Alejandro Labella, Department of Microbiology, University of Málaga. Málaga, Spain

Department of Microbiology, University of Málaga. Málaga, Spain

Manuel Manchado, IFAPA El Toruño Center, Junta de Andalucía, El Puerto de Santa María, Cádiz, Spain

IFAPA El Toruño Center, Junta de Andalucía, El Puerto de Santa María, Cádiz, Spain

M. Carmen Alonso, Department of Microbiology, University of Málaga. Málaga, Spain

Department of Microbiology, University of Málaga. Málaga, Spain

Dolores Castro, Department of Microbiology, University of Málaga. Málaga, Spain

Department of Microbiology, University of Málaga. Málaga, Spain

Juan J. Borrego, Department of Microbiology, University of Málaga. Málaga, Spain

Department of Microbiology, University of Málaga. Málaga, Spain

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Published

2010-01-22

Issue

Section

Research Articles