Influence of nutrients on enhancing laccase production by Botryosphaeria rhodina MAMB-05 Authors Robert F. H. Dekker Department of Biochemistry and Biotechnology-CCE, State University of Londrina, Londrina-PR, CEP 86051-990, Brazil University of Castilla-La Mancha, Regional Institute for Applied Research (IRICA), Ciudad Real, Spain Aneli M. Barbosa Department of Biochemistry and Biotechnology-CCE, State University of Londrina, Londrina-PR, CEP 86051-990, Brazil Ellen C. Giese Department of Biochemistry and Biotechnology-CCE, State University of Londrina, Londrina-PR, CEP 86051-990, Brazil Saulo D. S. Godoy Department of Biochemistry and Biotechnology-CCE, State University of Londrina, Londrina-PR, CEP 86051-990, Brazil Luiz G. Covizzi Department of Biochemistry and Biotechnology-CCE, State University of Londrina, Londrina-PR, CEP 86051-990, Brazil Keywords: Botryosphaeria rhodina, laccases, veratryl alcohol, C, N ratio and N sources, Tween and soybean oil, copper Abstract The physiological requirements needed to enhance the production of laccases by the ascomycete Botryosphaeria rhodina MAMB-05 in submerged cultivation were examined under non-induced and induced (veratryl alcohol, VA) conditions. Under non-induced conditions (–VA), the initial pH, C:N ratio, and inorganic N source did not influence laccase production, in contrast to Tween 80, soybean oil, and copper, which significantly increased laccase production, and proline and urea, which suppressed laccase formation. In addition, Tween 60 could serve as the sole carbon source for the production of these enzymes. Under VA-induced conditions of fungal growth, factors such as inoculum type, time-point of addition of inducer, initial pH, C:N ratio, and type of N source, influenced the production of laccases; however, unlike the non-induced conditions, proline and urea did not act as suppressors. Each of these physiological conditions exerted different effects on biomass production. The nutritional conditions examined for B. rhodina MAMB-05 are discussed in relation to their influence on fungal growth and laccase production. [Int Microbiol 2007; 10(3):177-185] Author Biographies Robert F. H. Dekker, Department of Biochemistry and Biotechnology-CCE, State University of Londrina, Londrina-PR, CEP 86051-990, Brazil University of Castilla-La Mancha, Regional Institute for Applied Research (IRICA), Ciudad Real, Spain Department of Biochemistry and Biotechnology-CCE, State University of Londrina, Londrina-PR, CEP 86051-990, Brazil University of Castilla-La Mancha, Regional Institute for Applied Research (IRICA), Ciudad Real, Spain Aneli M. Barbosa, Department of Biochemistry and Biotechnology-CCE, State University of Londrina, Londrina-PR, CEP 86051-990, Brazil Department of Biochemistry and Biotechnology-CCE, State University of Londrina, Londrina-PR, CEP 86051-990, Brazil Ellen C. Giese, Department of Biochemistry and Biotechnology-CCE, State University of Londrina, Londrina-PR, CEP 86051-990, Brazil Department of Biochemistry and Biotechnology-CCE, State University of Londrina, Londrina-PR, CEP 86051-990, Brazil Saulo D. S. Godoy, Department of Biochemistry and Biotechnology-CCE, State University of Londrina, Londrina-PR, CEP 86051-990, Brazil Department of Biochemistry and Biotechnology-CCE, State University of Londrina, Londrina-PR, CEP 86051-990, Brazil Luiz G. Covizzi, Department of Biochemistry and Biotechnology-CCE, State University of Londrina, Londrina-PR, CEP 86051-990, Brazil Department of Biochemistry and Biotechnology-CCE, State University of Londrina, Londrina-PR, CEP 86051-990, Brazil Downloads PDF Published 2010-01-22 Issue Vol. 10 No. 3 (2007) Section Research Articles License Submission of a manuscript to International Microbiology implies: that the work described has not been published before, including publication in the World Wide Web (except in the form of an Abstract or as part of a published lecture, review, or thesis); that it is not under consideration for publication elsewhere; that all the coauthors have agreed to its publication. 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