Colonization capacity and serum bactericidal activity of Haemophilus parasuis thy mutants Authors Anna Bigas Department of Genetics and Microbiology, Autonomous University of Barcelona, Bellaterra, Spain M. Elena Garrido Animal Health Research Center (CReSA), Autonomous University of Barcelona, and Institute for Agriculture and Food Technology Ignacio Badiola Animal Health Research Center (CReSA), Autonomous University of Barcelona, and Institute for Agriculture and Food Technology Jordi Barbé Department of Genetics and Microbiology, Autonomous University of Barcelona, Bellaterra, Spain; Animal Health Research Center (CReSA), Autonomous University of Barcelona, and Institute for Agriculture and Food Technology Montserrat Llagostera Department of Genetics and Microbiology, Autonomous University of Barcelona, Bellaterra, Spain; Animal Health Research Center (CReSA), Autonomous University of Barcelona, and Institute for Agriculture and Food Technology Keywords: Haemophilus parasuis, colonization capacity, bactericidal activity, thy mutants, vaccine strains, immunogenic response Abstract The bacterial thyA gene encodes the enzyme thymidylate synthase, which is essential for dTMP synthesis and, consequently, for DNA replication. In this work, a Haemophilus parasuis thyA mutant was constructed in order to analyze its colonization characteristics and its capacity to generate serum bactericidal activity in infected guinea pigs. The data showed that colonization by the H. parasuis thyA mutant was much less than that of the wild-type strain. Nevertheless, the mutant generated a strong immunogenic response in the host, as detected by measuring serum bactericidal activity. [Int Microbiol 2006; 9(4):297-301] Downloads PDF Published 2010-02-23 Issue Vol. 9 No. 4 (2006) Section Research Articles License Submission of a manuscript to International Microbiology implies: that the work described has not been published before, including publication in the World Wide Web (except in the form of an Abstract or as part of a published lecture, review, or thesis); that it is not under consideration for publication elsewhere; that all the coauthors have agreed to its publication. The corresponding author signs for and accepts responsability for releasing this material and will act on behalf of any and all coauthors regarding the editorial review and publication process.If an article is accepted for publication in International Microbiology, the authors (or other copyright holder) must transfer to the journal the right–not exclusive–to reproduce and distribute the article including reprints, translations, photographic reproductions, microform, electronic form (offline, online) or any other reproductions of similar nature. Nevertheless, all article in International Microbiology will be available on the Internet to any reader at no cost. The journal allows users to freely download, copy, print, distribute, search, and link to the full text of any article, provided the authorship and source of the published article is cited. The copyright owner's consent does not include copying for new works, or resale. In these cases, the specific written permission of International Microbiology must first be obtained.Authors are requested to create a link to the published article on the journal's website. The link must be accompanied by the following text: "The original publication is available on LINK at <http://www.im.microbios.org>. Please use the appropiate URL for the article in LINK. Articles disseminated via LINK are indexed, abstracted, and referenced by many abstracting and information services, bibliographic networks, subscription agencies, library networks, and consortia.
