Widespread occurrence of non-phosphorylating glyceraldehyde-3-phosphate dehydrogenase among gram-positive bacteria Authors Abdelghani Iddar Enzymatic Engineering and Molecular Genetics Team, Faculty of Sciences Aïn-Chock, University Hassan-II, Mâarif, Casablanca, Morocco Federico Valverde Institute for Plant Biochemistry and Photosynthesis (CSIC University of Seville), Seville, Spain Omar Assobhei Laboratory of Applied Microbiology and Biotechnology, Faculty of Sciences, Univ. Chouaib Doukkali, El Jadida, Morocco Aurelio Serrano Institute for Plant Biochemistry and Photosynthesis (CSIC University of Seville), Seville, Spain Abdelaziz Soukri Enzymatic Engineering and Molecular Genetics Team, Faculty of Sciences Aïn-Chock, University Hassan-II, Mâarif, Casablanca, Morocco Keywords: non-phosphorylating glyceraldehyde-3-phosphate dehydrogenase, Bacillus, Streptococcus, Clostridium, gapN genes Abstract The non-phosphorylating glyceraldehyde 3-phosphate dehydrogenase (GAPDHN, NADP+-specific, EC 1.2.1.9) is present in green eukaryotes and some Streptococcus strains. The present report describes the results of activity and immunoblot analyses, which were used to generate the first survey of bacterial GAPDHN distribution in a number of Bacillus, Streptococcus and Clostridium strains. Putative gapN genes were identified after PCR amplification of partial 700-bp sequences using degenerate primers constructed from highly conserved protein regions. Alignment of the amino acid sequences of these fragments with those of known sequences from other eukaryotic and prokaryotic GAPDHNs, demonstrated the presence of conserved residues involved in catalytic activity that are not conserved in aldehyde dehydrogenases, a protein family closely linked to GAPDHNs. The results confirm that the basic structural features of the members of the GAPDHN family have been conserved throughout evolution and that no identity exists with phosphorylating GAPDHs. Furthermore, phylogenetic trees generated from multiple sequence alignments suggested a close relationship between plant and bacterial GAPDHN families. [Int Microbiol 2005; 8(4):251-258] Downloads PDF Published 2010-02-26 Issue Vol. 8 No. 4 (2005) Section Research Articles License Submission of a manuscript to International Microbiology implies: that the work described has not been published before, including publication in the World Wide Web (except in the form of an Abstract or as part of a published lecture, review, or thesis); that it is not under consideration for publication elsewhere; that all the coauthors have agreed to its publication. The corresponding author signs for and accepts responsability for releasing this material and will act on behalf of any and all coauthors regarding the editorial review and publication process.If an article is accepted for publication in International Microbiology, the authors (or other copyright holder) must transfer to the journal the right–not exclusive–to reproduce and distribute the article including reprints, translations, photographic reproductions, microform, electronic form (offline, online) or any other reproductions of similar nature. Nevertheless, all article in International Microbiology will be available on the Internet to any reader at no cost. The journal allows users to freely download, copy, print, distribute, search, and link to the full text of any article, provided the authorship and source of the published article is cited. The copyright owner's consent does not include copying for new works, or resale. In these cases, the specific written permission of International Microbiology must first be obtained.Authors are requested to create a link to the published article on the journal's website. The link must be accompanied by the following text: "The original publication is available on LINK at <http://www.im.microbios.org>. Please use the appropiate URL for the article in LINK. Articles disseminated via LINK are indexed, abstracted, and referenced by many abstracting and information services, bibliographic networks, subscription agencies, library networks, and consortia.
