Peculiarities of the DNA of MM1, a temperate phage of Streptococcus pneumoniae Authors Virginia Obregón Department of Molecular Biology, Biological Research Center, CSIC, Madrid, Spain. Present address: Bioferma, Cartagena (Murcia), Spain José L. García Department of Molecular Biology, Biological Research Center, CSIC, Madrid, Spain Ernesto García Department of Molecular Biology, Biological Research Center, CSIC, Madrid, Spain Rubens López Department of Molecular Biology, Biological Research Center, CSIC, Madrid, Spain Pedro García Department of Molecular Biology, Biological Research Center, CSIC, Madrid, Spain Keywords: pneumococcus, DNA-protein complex, pac site, temperate phages Abstract The abundant presence of temperate phages in the chromosomes of clinical isolates of Streptococcus pneumoniae has been well documented. The genome of MM1, a temperate phage of pneumococcus, has been isolated as a DNA-protein complex. The protein is covalently bound to the DNA, was iodinated in vitro with Na125I, and has an Mr of 22,000. Electron microscopy and enzymatic analyses revealed that the MM1 genome is a linear, circularly permuted, terminally redundant collection of double-stranded DNA molecules packaged via a headful mechanism. The location of the pac site appears to be downstream of the terminase, between orf32 and orf34 of the MM1 genome. [Int Microbiol 2004; 7(2):133-137] Downloads PDF Published 2010-02-28 Issue Vol. 7 No. 2 (2004) Section Research Articles License Submission of a manuscript to International Microbiology implies: that the work described has not been published before, including publication in the World Wide Web (except in the form of an Abstract or as part of a published lecture, review, or thesis); that it is not under consideration for publication elsewhere; that all the coauthors have agreed to its publication. The corresponding author signs for and accepts responsability for releasing this material and will act on behalf of any and all coauthors regarding the editorial review and publication process.If an article is accepted for publication in International Microbiology, the authors (or other copyright holder) must transfer to the journal the right–not exclusive–to reproduce and distribute the article including reprints, translations, photographic reproductions, microform, electronic form (offline, online) or any other reproductions of similar nature. Nevertheless, all article in International Microbiology will be available on the Internet to any reader at no cost. The journal allows users to freely download, copy, print, distribute, search, and link to the full text of any article, provided the authorship and source of the published article is cited. The copyright owner's consent does not include copying for new works, or resale. In these cases, the specific written permission of International Microbiology must first be obtained.Authors are requested to create a link to the published article on the journal's website. The link must be accompanied by the following text: "The original publication is available on LINK at <http://www.im.microbios.org>. Please use the appropiate URL for the article in LINK. Articles disseminated via LINK are indexed, abstracted, and referenced by many abstracting and information services, bibliographic networks, subscription agencies, library networks, and consortia.
