Clonal spread of Klebsiella pneumoniae producing OXA-1 betalactamase in a Spanish hospital

Authors

  • Meritxell Cubero Microbiology Department and 3Infectious Diseases Department, University Hospital of Bellvitge-University of Barcelona- IDIBELL, Barcelona.
  • Laura Calatayud Microbiology Department and 3Infectious Diseases Department, University Hospital of Bellvitge-University of Barcelona- IDIBELL, Barcelona.
  • Fe Tubau Microbiology Department and 3Infectious Diseases Department, University Hospital of Bellvitge-University of Barcelona- IDIBELL, Barcelona.
  • Josefina Ayats Microbiology Department and 3Infectious Diseases Department, University Hospital of Bellvitge-University of Barcelona- IDIBELL, Barcelona.
  • Carmen Peña Infectious Diseases Department, University Hospital of Bellvitge-University of Barcelona- IDIBELL, Barcelona, Spain.
  • Rogelio Martín Microbiology Department and 3Infectious Diseases Department, University Hospital of Bellvitge-University of Barcelona- IDIBELL, Barcelona.
  • Josefina Liñares Microbiology Department and 3Infectious Diseases Department, University Hospital of Bellvitge-University of Barcelona- IDIBELL, Barcelona.
  • M. Ángeles Domínguez Microbiology Department and 3Infectious Diseases Department, University Hospital of Bellvitge-University of Barcelona- IDIBELL, Barcelona.
  • Carmen Ardanuy Microbiology Department and 3Infectious Diseases Department, University Hospital of Bellvitge-University of Barcelona- IDIBELL, Barcelona.

Keywords:

Klebsiella pneumoniae, sequence type ST14, gene blaOXA-1, integrons, nosocomial outbreaks

Abstract

Multi-drug resistant Klebsiella pneumoniae isolates are associated with nosocomial infections, in which colonized patients act as a reservoir and source of cross-infection for other patients. In this study, the antimicrobial susceptibility of K. pneumoniae was tested by microdilution using the commercial method MicroScan (Siemens). The genetic relatedness of K. pneumoniae strains was determined by pulsed field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). PCR experiments were carried out to obtain primer sets and positive PCR products were purified and sequenced. From May 2007 until December 2009, 98 clonally related K. pneumoniae isolates were detected from clinical samples of 38 patients admitted to the University Hospital of Bellvitge, Barcelona, Spain, including 27 admitted to the intensive care unit (ICU). The most important sources of the isolates were: lower respiratory tract (n = 12), urine (n = 12), and blood (n = 11). The strains were resistant to amoxicillin/clavulanic acid, piperacillin/tazobactam, tobramycin, amikacin, and ciprofloxacin, and had diminished susceptibility to cefepime. All the isolates shared a common PFGE pattern related to sequence type 14 after MLST analysis. In K. pneumoniae isolates and their transconjugants, the blaOXA-1 gene was located in the variable region of a class I integron that also contains the aac(6′)Ib-cr gene. Sequencing of the quinolone resistance determinant regions of gyrA and parC revealed a S83F change in GyrA and no changes in ParC. [Int Microbiol 2013; 16(4):227-233]

Keywords: Klebsiella pneumoniae · sequence type ST14 · gene blaOXA-1 · integrons · nosocomial outbreaks

Author Biography

Meritxell Cubero, Microbiology Department and 3Infectious Diseases Department, University Hospital of Bellvitge-University of Barcelona- IDIBELL, Barcelona.



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Issue

Vol. 16 No. 4 (2013)

Section

Research Articles

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