Bacterial membrane injuries induced by lactacin F and nisin

Authors

  • M. Dalmau Biomedical Research Center of Bellvitge, Laboratories of Microbiology and Flow Cytometry, University of Barcelona, Campus de Bellvitge,
  • Elke Maier Lehrstuhlfür Biotechnologie, Theodor-Boveri-Institut (Biozentrum) der Universität Würzburg, Am Hubland, Würzburg, Germany
  • Natalia Mulet Biomedical Research Center of Bellvitge, Laboratories of Microbiology and Flow Cytometry, University of Barcelona, Campus de Bellvitge,
  • Miguel Viñas Biomedical Research Center of Bellvitge, Laboratories of Microbiology and Flow Cytometry, University of Barcelona, Campus de Bellvitge,
  • Roland Benz Lehrstuhlfür Biotechnologie, Theodor-Boveri-Institut (Biozentrum) der Universität Würzburg, Am Hubland, Würzburg, Germany

Keywords:

lactacin F, nisin, lactic acid bacteria (lab), planar lipid bilayer, flow cytometry

Abstract

The combined action of nisin and lactacin F, two bacteriocins produced by lactic acid bacteria, is additive. In this report, the basis of this effect is examined. Channels formed by lactacin F were studied by experiments using planar lipid bilayers, and bactericidal effects were analyzed by flow cytometry. Lactacin F produced pores with a conductance of 1 ns in black lipid bilayers in 1 mM KClat 10 mV at 20°C. Pore formation was strongly dependent on voltage. Although lactacin F formed pores at very low potential (10 mV), the dependence was exponentialabov e 40 mV. The injuries induced by nisin and lactacin F in the membranes of Lactobacillus helveticus produced different flow cytometric profiles. Probably, when both bacteriocins are present, each acts separately; their cooperation may be due to an increase in the number of single membrane injuries.

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Published

2010-03-10

Issue

Vol. 5 No. 2 (2002)

Section

Research Articles

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