Effect of the fungicide Prochloraz-Mn on the cell wall structure of Verticillium fungicola

Authors

  • D. Bernardo Centro de Investigaciones Biológicas, CSIC, Madrid, Spain
  • M. Novaes-Ledieu Centro de Investigaciones Biológicas, CSIC, Madrid, Spain
  • A. Pérez Cabo Centro de Investigaciones Biológicas, CSIC, Madrid, Spain
  • F. J. Gea Alegría Centro de Investigación, Experimentación y Servicios del Champiñón, Quintanar del Rey, Cuenca, Spain
  • C. García Mendoza Centro de Investigaciones Biológicas, CSIC, Madrid, Spain

Keywords:

Verticillium fungicola, cell wall, partial protein inhibition, carbohydrate rearrangement, Prochloraz-Mn

Abstract

The chemical structure of the cell wall of two isolates of Verticillium fungicola collected from diseased fruit bodies of the commercial mushroom Agaricus bisporus treated with the fungicide Prochloraz-Mn was analyzed. The isolates were obtained during different periods of time and grown in the absence and presence of the LD50 values of the fungicide for V. fungicola. In addition, another V. fungicola isolate collected previous to the routine utilization of Prochloraz-Mn but grown under the same conditions was also analyzed. The overall chemical composition of the cell wall from the three isolates showed detectable differences in their basic components, with a significant decrease in the protein content in fungicide-treated cells. This inhibitory effect was partially compensated by an increase in neutral and/or aminated carbohydrates and was accompanied by appreciable modifications of polysaccharide structure, as deduced after methylation analysis and gas-liquid chromatography- mass spectrometry (GLC-MS). Moreover, differences in hyphal morphology caused by the fungicide were observed by transmission electron microscopy (TEM).

Author Biographies

D. Bernardo, Centro de Investigaciones Biológicas, CSIC, Madrid, Spain

Centro de Investigaciones Biológicas, CSIC, Madrid, Spain

M. Novaes-Ledieu, Centro de Investigaciones Biológicas, CSIC, Madrid, Spain

Centro de Investigaciones Biológicas, CSIC, Madrid, Spain

A. Pérez Cabo, Centro de Investigaciones Biológicas, CSIC, Madrid, Spain

Centro de Investigaciones Biológicas, CSIC, Madrid, Spain

F. J. Gea Alegría, Centro de Investigación, Experimentación y Servicios del Champiñón, Quintanar del Rey, Cuenca, Spain

Centro de Investigación, Experimentación y Servicios del Champiñón, Quintanar del Rey, Cuenca, Spain

C. García Mendoza, Centro de Investigaciones Biológicas, CSIC, Madrid, Spain

Centro de Investigaciones Biológicas, CSIC, Madrid, Spain

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Published

2010-09-21

Issue

Vol. 5 No. 3 (2002)

Section

Research Articles

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