DNA recognition by the Salmonella enterica serovar Typhimurium transcription factor SlyA Authors Fouzia Haider Department of Molecular Biology and Biotechnology, University of Sheffield James K. Lithgow Department of Molecular Biology and Biotechnology, University of Sheffield Melanie R. Stapleton Department of Molecular Biology and Biotechnology, University of Sheffield Valia A. Norte Department of Molecular Biology and Biotechnology, University of Sheffield Ruth E. Roberts Department of Molecular Biology and Biotechnology, University of Sheffield Jefrrey Green Department of Molecular Biology and Biotechnology, University of Sheffield Keywords: SlyA, gene regulation, DNA binding, MarR, OhrR Abstract The Salmonella regulatory protein, SlyA is implicated in virulence, survival in macrophages and resistance to oxidative stress and anti-microbial peptides. SlyA is a member of the MarR family of winged-helix transcription factors. Systematic mutational analysis of the SlyA operator sequence and of the predicted DNA-binding region of SlyA shows that no single base pair in the palindromic SlyA operator sequence is essential for DNA binding, and identifies amino acid residues required to allow SlyA to recognise DNA. Combining the structure-function studies described here and elsewhere with the structures of MarR family proteins suggests a possible model for regulation of SlyA binding to DNA. Author Biographies Fouzia Haider, Department of Molecular Biology and Biotechnology, University of Sheffield Department of Molecular Biology and Biotechnology, University of Sheffield James K. Lithgow, Department of Molecular Biology and Biotechnology, University of Sheffield Department of Molecular Biology and Biotechnology, University of Sheffield Melanie R. Stapleton, Department of Molecular Biology and Biotechnology, University of Sheffield Department of Molecular Biology and Biotechnology, University of Sheffield Valia A. Norte, Department of Molecular Biology and Biotechnology, University of Sheffield Department of Molecular Biology and Biotechnology, University of Sheffield Ruth E. Roberts, Department of Molecular Biology and Biotechnology, University of Sheffield Department of Molecular Biology and Biotechnology, University of Sheffield Jefrrey Green, Department of Molecular Biology and Biotechnology, University of Sheffield Department of Molecular Biology and Biotechnology, University of Sheffield Downloads PDF Published 2010-09-21 Issue Vol. 11 No. 4 (2008) Section Research Articles License Submission of a manuscript to International Microbiology implies: that the work described has not been published before, including publication in the World Wide Web (except in the form of an Abstract or as part of a published lecture, review, or thesis); that it is not under consideration for publication elsewhere; that all the coauthors have agreed to its publication. The corresponding author signs for and accepts responsability for releasing this material and will act on behalf of any and all coauthors regarding the editorial review and publication process.If an article is accepted for publication in International Microbiology, the authors (or other copyright holder) must transfer to the journal the right–not exclusive–to reproduce and distribute the article including reprints, translations, photographic reproductions, microform, electronic form (offline, online) or any other reproductions of similar nature. Nevertheless, all article in International Microbiology will be available on the Internet to any reader at no cost. The journal allows users to freely download, copy, print, distribute, search, and link to the full text of any article, provided the authorship and source of the published article is cited. The copyright owner's consent does not include copying for new works, or resale. In these cases, the specific written permission of International Microbiology must first be obtained.Authors are requested to create a link to the published article on the journal's website. The link must be accompanied by the following text: "The original publication is available on LINK at <http://www.im.microbios.org>. Please use the appropiate URL for the article in LINK. Articles disseminated via LINK are indexed, abstracted, and referenced by many abstracting and information services, bibliographic networks, subscription agencies, library networks, and consortia.
